Hotspot

trnT

LSC · 16565-16636 · 72 bp

Back to Tripsacum

Pi 0.0139

MarkerSeek score 60.50

Primer available yes

Label trnT

Feature Metadata

label_name: trnT
feature_type: tRNA
region: LSC
length_bp: 72
variable_sites: 1
pi: 0.0139
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT_p1	AGCCCTTTTAACTCAGTGGT	CCAAAAATGTGGGCATCTCG	45.0	50.0	56.7	57.7	238-239 bp	1.0	67.6
trnT_p2	AGGCATAAGTCATCGGTTCA	CCAAAAATGTGGGCATCTCG	45.0	50.0	56.6	57.7	201-202 bp	1.0	67.6
trnT_p3	AGCCCTTTTAACTCAGTGGT	CAAAAATGTGGGCATCTCGC	45.0	50.0	56.7	58.4	237-238 bp	1.0	66.9
trnT_p4	AGGCATAAGTCATCGGTTCA	CAAAAATGTGGGCATCTCGC	45.0	50.0	56.6	58.4	200-201 bp	1.0	66.9
trnT_p5	TGGTAAGGCATAAGTCATCGG	CCAAAAATGTGGGCATCTCG	47.6	50.0	57.5	57.7	206-207 bp	1.0	66.8