Hotspot

petD

LSC · 75354-76579 · 1226 bp

Back to Tripsacum

Pi 0.00245

MarkerSeek score 60.20

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1226
variable_sites: 3
pi: 0.00245
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	ACGAAAGAAAATAAGGCGGA	TTCGCGGTGGAATGAATCAA	40.0	45.0	55.4	58.2	1285 bp	1.0	57.7
petD_p2	ACGAAAGAAAATAAGGCGGAT	TTCGCGGTGGAATGAATCAA	38.1	45.0	56.0	58.2	1285 bp	1.0	54.3
petD_p3	ACGAAAGAAAATAAGGCGGA	ACTTCGCGGTGGAATGAATC	40.0	50.0	55.4	58.6	1287 bp	1.0	54.2
petD_p4	ACGAAAGAAAATAAGGCGGA	GACTTCGCGGTGGAATGAAT	40.0	50.0	55.4	58.6	1288 bp	1.0	54.2
petD_p5	ACGAAAGAAAATAAGGCGGA	CTTCGCGGTGGAATGAATCA	40.0	50.0	55.4	58.6	1286 bp	1.0	54.2