Hotspot

infA

LSC · 78720-79043 · 324 bp

Back to Tripsacum

Pi 0.00309

MarkerSeek score 54.80

Primer available yes

Label infA

Feature Metadata

label_name: infA
feature_type: gene
region: LSC
length_bp: 324
variable_sites: 1
pi: 0.00309
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
infA_p1	ACTTGGAAGTTATTACCCCAGA	ATGGCCCCACCTATAGTACC	40.9	55.0	56.4	58.0	431 bp	1.0	51.4
infA_p2	ACTTGGAAGTTATTACCCCAGA	ATATGGTAATGGCCCCACCT	40.9	50.0	56.4	58.2	439 bp	1.0	50.5
infA_p3	ACTTGGAAGTTATTACCCCAGA	ATGGTAATGGCCCCACCTAT	40.9	50.0	56.4	58.2	437 bp	1.0	50.5
infA_p4	ACTTGGAAGTTATTACCCCAGA	GCCCCACCTATAGTACCCAA	40.9	55.0	56.4	58.2	428 bp	1.0	50.2
infA_p5	ACTTGGAAGTTATTACCCCAGAA	ATGGCCCCACCTATAGTACC	39.1	55.0	57.1	58.0	431 bp	1.0	49.0