Hotspot

ndhF-rpl32

SSC · 107935-108786 · 852 bp

Back to Tripsacum

Pi 0.00236
MarkerSeek score 60.80
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
852
variable_sites
2
pi
0.00236
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 CTGGAAGTGGGAGAAGAGGA TCCAAAGATTTTTACGAATACGCT 55.0 33.3 58.1 57.3 965-980 bp 1.0 42.4
ndhF-rpl32_p2 CTGGAAGTGGGAGAAGAGGA TGACATCGAAGTACGTTTTT 55.0 35.0 58.1 53.2 937-952 bp 1.0 42.1
ndhF-rpl32_p3 CTGGAAGTGGGAGAAGAGGA TTGACATCGAAGTACGTTTT 55.0 35.0 58.1 53.2 938-953 bp 1.0 42.1
ndhF-rpl32_p4 CTGGAAGTGGGAGAAGAGGA TTTGACATCGAAGTACGTTT 55.0 35.0 58.1 53.2 939-954 bp 1.0 42.1
ndhF-rpl32_p5 CTGGAAGTGGGAGAAGAGGA TTTTGACATCGAAGTACGTT 55.0 35.0 58.1 53.2 940-955 bp 1.0 42.1