Hotspot

rpl20-rps12

LSC · 69279-69962 · 684 bp

Back to Tripsacum

Pi 0.00147
MarkerSeek score 60.30
Primer available yes
Label rpl20-rps12

Feature Metadata

label_name
rpl20-rps12
feature_type
igs
region
LSC
length_bp
684
variable_sites
1
pi
0.00147
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl20-rps12_p1 ACGCATTTTTGTTCGTCGTC GAGGAACATGTGCTAGGGTG 45.0 55.0 58.0 58.0 756-761 bp 1.0 80.3
rpl20-rps12_p2 ACGCATTTTTGTTCGTCGTC CGACATGCCAACGGTTAAAC 45.0 50.0 58.0 58.0 851-856 bp 1.0 80.1
rpl20-rps12_p3 ACGCATTTTTGTTCGTCGTC GTCGAGGAACATGTGCTAGG 45.0 55.0 58.0 58.1 759-764 bp 1.0 79.9
rpl20-rps12_p4 GTTTGAGGCAAATGAACGCA GAGGAACATGTGCTAGGGTG 45.0 55.0 57.9 58.0 771-776 bp 1.0 79.3
rpl20-rps12_p5 GTTTGAGGCAAATGAACGCA CGACATGCCAACGGTTAAAC 45.0 50.0 57.9 58.0 866-871 bp 1.0 79.2