Hotspot

rpl33-rps18

LSC · 67879-68183 · 305 bp

Back to Tripsacum

Pi 0.00984

MarkerSeek score 47.60

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 305
variable_sites: 3
pi: 0.00984
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	AAAGGAGGTTCATGGCCAAG	TCGAGAACGCCTACGAAAAG	50.0	50.0	58.1	58.0	682-698 bp	1.0	79.7
rpl33-rps18_p2	GAAAGGAGGTTCATGGCCAA	TCGAGAACGCCTACGAAAAG	50.0	50.0	58.1	58.0	683-699 bp	1.0	79.6
rpl33-rps18_p3	AAAGGAGGTTCATGGCCAAG	AACGCCTACGAAAAGGTTGT	50.0	45.0	58.1	58.0	677-693 bp	1.0	79.6
rpl33-rps18_p4	AAAGGAGGTTCATGGCCAAG	ACGCCTACGAAAAGGTTGTT	50.0	45.0	58.1	58.0	676-692 bp	1.0	79.6
rpl33-rps18_p5	GAAAGGAGGTTCATGGCCAA	ACGCCTACGAAAAGGTTGTT	50.0	45.0	58.1	58.0	677-693 bp	1.0	79.5