Hotspot

petA-psbJ

LSC · 63116-64030 · 915 bp

Back to Tripsacum

Pi 0.0011

MarkerSeek score 60.40

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 915
variable_sites: 1
pi: 0.0011
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CGTCCAAGGCCTTTTGTTTT	ACGGTTCGTATTCTGGATTGG	45.0	47.6	57.4	58.1	1049-1059 bp	1.0	67.3
petA-psbJ_p2	CGTCCAAGGCCTTTTGTTTT	GGTTCGTATTCTGGATTGGGT	45.0	47.6	57.4	57.7	1047-1057 bp	1.0	65.9
petA-psbJ_p3	CGTCCAAGGCCTTTTGTTTTT	ACGGTTCGTATTCTGGATTGG	42.9	47.6	58.1	58.1	1049-1059 bp	1.0	63.2
petA-psbJ_p4	CGTCCAAGGCCTTTTGTTTT	CGGTTCGTATTCTGGATTGG	45.0	50.0	57.4	56.3	1048-1058 bp	1.0	62.6
petA-psbJ_p5	AGGCCTTTTGTTTTTCTTCGC	ACGGTTCGTATTCTGGATTGG	42.9	47.6	58.2	58.1	1043-1053 bp	1.0	62.6