Hotspot

trnT-trnE

LSC · 16637-17097 · 461 bp

Back to Tripsacum

Pi 0

MarkerSeek score 45.70

Primer available yes

Label trnT-trnE

Feature Metadata

label_name: trnT-trnE
feature_type: igs
region: LSC
length_bp: 461
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-trnE_p1	GACAGAAGTCATCGGTTCGA	GCTGCCTCCTTGAAAGAGAG	50.0	55.0	57.4	58.3	543-544 bp	1.0	73.0
trnT-trnE_p2	GACAGAAGTCATCGGTTCGA	TGTCCTGAACCACTAGACGA	50.0	50.0	57.4	57.7	522-523 bp	1.0	72.8
trnT-trnE_p3	GACAGAAGTCATCGGTTCGA	CCTGAACCACTAGACGATGG	50.0	55.0	57.4	57.4	519-520 bp	1.0	70.4
trnT-trnE_p4	GACAGAAGTCATCGGTTCGA	CTGAACCACTAGACGATGGG	50.0	55.0	57.4	57.4	518-519 bp	1.0	70.4
trnT-trnE_p5	GACAGAAGTCATCGGTTCGAA	GCTGCCTCCTTGAAAGAGAG	47.6	55.0	58.1	58.3	543-544 bp	1.0	69.6