Hotspot

trnG-trnT

LSC · 14547-16497 · 1951 bp

Back to Tripsacum

Pi 0.00103

MarkerSeek score 49.30

Primer available yes

Label trnG-trnT

Feature Metadata

label_name: trnG-trnT
feature_type: igs
region: LSC
length_bp: 1951
variable_sites: 2
pi: 0.00103
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-trnT_p1	ACGAATCACACTTTTACCACT	TGAACCGATGACTTATGCCT	38.1	45.0	55.5	56.6	2041-2046 bp	1.0	41.0
trnG-trnT_p2	ACGAATCACACTTTTACCACT	CCGATGACTTATGCCTTACCA	38.1	47.6	55.5	57.5	2037-2042 bp	1.0	40.3
trnG-trnT_p3	ACGAATCACACTTTTACCACT	ACCGATGACTTATGCCTTACC	38.1	47.6	55.5	57.5	2038-2043 bp	1.0	40.3
trnG-trnT_p4	AGAACGAATCACACTTTTACCAC	TGAACCGATGACTTATGCCT	39.1	45.0	57.3	56.6	2044-2049 bp	1.0	40.3
trnG-trnT_p5	GAACGAATCACACTTTTACCACT	TGAACCGATGACTTATGCCT	39.1	45.0	57.3	56.6	2043-2048 bp	1.0	40.3