Hotspot

rps16

LSC · 4476-5477 · 1002 bp

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Pi 0

MarkerSeek score 40.00

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1002
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	TTGTTGGATTGGCACCACAT	TGGATCAATTCTCGATACCACT	45.0	40.9	58.3	56.7	1166-1167 bp	1.0	51.3
rps16_p2	GCACCACATAAATGCAGCAA	TGGATCAATTCTCGATACCACT	45.0	40.9	57.6	56.7	1155-1156 bp	1.0	50.7
rps16_p3	TTTGTTGGATTGGCACCACA	TGGATCAATTCTCGATACCACT	45.0	40.9	58.5	56.7	1167-1168 bp	1.0	49.5
rps16_p4	TGTTGGATTGGCACCACATA	TGGATCAATTCTCGATACCACT	45.0	40.9	57.4	56.7	1165-1166 bp	1.0	48.9
rps16_p5	TTGTTGGATTGGCACCACAT	TGGATCAATTCTCGATACCACTT	45.0	39.1	58.3	57.4	1166-1167 bp	1.0	48.8