Hotspot

rpl16

LSC · 76259-77756 · 1498 bp

Back to Tripogon

Pi 0.000668

MarkerSeek score 59.10

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1498
variable_sites: 1
pi: 0.000668
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	GCTCCACTGTTATCCGCTAC	AGACTTATTTTCCAAAAATCCCAGA	55.0	32.0	58.1	56.9	1710 bp	1.0	40.0
rpl16_p2	GCTCCACTGTTATCCGCTAC	AGAGACTTATTTTCCAAAAATCCCA	55.0	32.0	58.1	56.9	1712 bp	1.0	40.0
rpl16_p3	GCTCCACTGTTATCCGCTAC	GAGACTTATTTTCCAAAAATCCCAGA	55.0	34.6	58.1	57.9	1711 bp	1.0	40.0
rpl16_p4	GCTCCACTGTTATCCGCTAC	AGAGACTTATTTTCCAAAAATCCCAG	55.0	34.6	58.1	57.9	1712 bp	1.0	40.0
rpl16_p5	AGCTCCACTGTTATCCGCTA	AGACTTATTTTCCAAAAATCCCAGA	50.0	32.0	58.2	56.9	1711 bp	1.0	40.0