Hotspot

rpl32-trnL-UAG

SSC · 103401-103919 · 519 bp

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Pi 0.00193
MarkerSeek score 58.20
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
519
variable_sites
1
pi
0.00193
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GCAACGAGCATTCAAAACCA TCCTAAGAGCAGCGTGTCTA 45.0 50.0 57.9 57.9 596 bp 1.0 78.1
rpl32-trnL-UAG_p2 GCAACGAGCATTCAAAACCA AGCAGCGTGTCTACCAATTT 45.0 45.0 57.9 57.8 589 bp 1.0 77.6
rpl32-trnL-UAG_p3 GCAACGAGCATTCAAAACCA GCTTGAGCACTGCTTCCTAA 45.0 50.0 57.9 58.2 610 bp 1.0 77.6
rpl32-trnL-UAG_p4 GCAACGAGCATTCAAAACCA CCTAAGAGCAGCGTGTCTAC 45.0 55.0 57.9 57.8 595 bp 1.0 77.5
rpl32-trnL-UAG_p5 GCAACGAGCATTCAAAACCA CTAAGAGCAGCGTGTCTACC 45.0 55.0 57.9 57.8 594 bp 1.0 77.5