Hotspot

trnP-UGG-psaJ

LSC · 62941-63309 · 369 bp

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Pi 0.00271

MarkerSeek score 55.50

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 369
variable_sites: 1
pi: 0.00271
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	AAAACAAACGCGCTACCAAG	CCGCATGGAAAATCCTCCTT	45.0	50.0	57.9	58.2	440 bp	1.0	77.4
trnP-UGG-psaJ_p2	CAAAACAAACGCGCTACCAA	CCGCATGGAAAATCCTCCTT	45.0	50.0	58.2	58.2	441 bp	1.0	77.0
trnP-UGG-psaJ_p3	AAAACAAACGCGCTACCAAG	ACCTGCTAAAACCCCAAACC	45.0	50.0	57.9	58.3	506 bp	1.0	76.9
trnP-UGG-psaJ_p4	AAAACAAACGCGCTACCAAG	CCTGCTAAAACCCCAAACCA	45.0	50.0	57.9	58.3	505 bp	1.0	76.9
trnP-UGG-psaJ_p5	TTGTACCCAAAACAAACGCG	CCGCATGGAAAATCCTCCTT	45.0	50.0	57.8	58.2	448 bp	1.0	76.8