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matK-trnK-UUU

LSC · 3293-3894 · 602 bp

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Pi 0

MarkerSeek score 40.00

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 602
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	ACCCTTTGAATTTTGCCATTTGT	CCCGGTGTAATTCCATCGAT	34.8	50.0	58.2	57.5	775 bp	1.0	50.0
matK-trnK-UUU_p2	ACCCTTTGAATTTTGCCATTTGT	ATGTTTTCTCAGGAACCCCC	34.8	50.0	58.2	57.4	792 bp	1.0	49.5
matK-trnK-UUU_p3	ACCCTTTGAATTTTGCCATTTG	CCCGGTGTAATTCCATCGAT	36.4	50.0	56.5	57.5	775 bp	1.0	48.0
matK-trnK-UUU_p4	CCCTTTGAATTTTGCCATTTGT	CCCGGTGTAATTCCATCGAT	36.4	50.0	56.5	57.5	774 bp	1.0	48.0
matK-trnK-UUU_p5	ACGAAGTATTGTTGACGAGA	CCCGGTGTAATTCCATCGAT	40.0	50.0	54.5	57.5	812 bp	1.0	47.6