Hotspot

ycf2

IRa · 150100-157290 · 7191 bp

Back to Triplostegia

Pi 0.00536

MarkerSeek score 44.70

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: IRa
length_bp: 7191
variable_sites: 37
pi: 0.00536
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 44.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	AAATGGTAGACACGCGAGAC	GAACCCTGGAGATCCACTCT	50.0	55.0	58.0	58.1	1053-1202 bp	1.0	90.5
ycf2_p2	TGAAATGGTAGACACGCGAG	GAACCCTGGAGATCCACTCT	50.0	55.0	58.0	58.1	1055-1204 bp	1.0	90.5
ycf2_p3	AAATGGTAGACACGCGAGAC	CAAGAGGGTGGTGGAGAGTA	50.0	55.0	58.0	58.1	1202-1383 bp	1.0	90.1
ycf2_p4	TGAAATGGTAGACACGCGAG	CAAGAGGGTGGTGGAGAGTA	50.0	55.0	58.0	58.1	1204-1385 bp	1.0	90.0
ycf2_p5	GCCTTGGTGGTGAAATGGTA	CAAGAGGGTGGTGGAGAGTA	50.0	55.0	58.1	58.1	1214-1395 bp	1.0	89.4