Hotspot

ycf1

SSC · 127868-133333 · 5466 bp

Back to Triplostegia

Pi 0.0534

MarkerSeek score 82.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5466
variable_sites: 245
pi: 0.0534
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 82.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GGAGAAGCAGGTTCGCAATA	TGCCGAAGTGATGGAAAACA	50.0	45.0	58.0	58.0	925-1132 bp	1.0	90.0
ycf1_p2	CAGGTTTTCCCAGGCTTCTT	TGCCGAAGTGATGGAAAACA	50.0	45.0	58.0	58.0	1146-1376 bp	1.0	89.0
ycf1_p3	CAGGTTTTCCCAGGCTTCTT	CATATCCGCCCAGTTTGTCA	50.0	50.0	58.0	58.0	1112-1342 bp	1.0	89.0
ycf1_p4	CAGGTTTTCCCAGGCTTCTT	ACATATCCGCCCAGTTTGTC	50.0	50.0	58.0	58.0	1113-1343 bp	1.0	89.0
ycf1_p5	CAGGTTTTCCCAGGCTTCTT	AGTCAAGAATGGAATCGCCC	50.0	50.0	58.0	58.0	1239-1469 bp	1.0	88.5