Hotspot

rpl22

LSC · 87144-87746 · 603 bp

Back to Triplostegia

Pi 0.0149

MarkerSeek score 67.00

Primer available yes

Label rpl22

Feature Metadata

label_name: rpl22
feature_type: gene
region: LSC
length_bp: 603
variable_sites: 7
pi: 0.0149
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.78
markerseek_score: 67.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22_p1	GTTGTGCAAACCAAAGGGAA	TCGCGTCGTTAAGAAATGTT	45.0	40.0	57.3	56.1	632-764 bp	1.0	64.3
rpl22_p2	GTTGTGCAAACCAAAGGGAA	GATCGCGTCGTTAAGAAATGT	45.0	42.9	57.3	57.1	634-766 bp	1.0	64.1
rpl22_p3	TGTGCAAACCAAAGGGAATG	TCGCGTCGTTAAGAAATGTT	45.0	40.0	57.1	56.1	630-762 bp	1.0	62.5
rpl22_p4	GTTGTGCAAACCAAAGGGAA	ATCGCGTCGTTAAGAAATGT	45.0	40.0	57.3	55.9	633-765 bp	1.0	62.5
rpl22_p5	TGTGCAAACCAAAGGGAATG	GATCGCGTCGTTAAGAAATGT	45.0	42.9	57.1	57.1	632-764 bp	1.0	62.3