Hotspot

trnL-CAA-ycf2

IRa · 149477-150099 · 623 bp

Back to Triplostegia

Pi 0.168

MarkerSeek score 80.30

Primer available yes

Label trnL-CAA-ycf2

Feature Metadata

label_name: trnL-CAA-ycf2
feature_type: igs
region: IRa
length_bp: 623
variable_sites: 87
pi: 0.168
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-CAA-ycf2_p1	AAATGGTAGACACGCGAGAC	TGGCTTTTCCCGGATGAAAA	50.0	45.0	58.0	58.0	719-765 bp	1.0	91.9
trnL-CAA-ycf2_p2	TGAAATGGTAGACACGCGAG	TGGCTTTTCCCGGATGAAAA	50.0	45.0	58.0	58.0	721-767 bp	1.0	91.9
trnL-CAA-ycf2_p3	CCAACACTCCACCTTTGTCA	TGGCTTTTCCCGGATGAAAA	50.0	45.0	57.9	58.0	813-859 bp	1.0	91.6
trnL-CAA-ycf2_p4	AAATGGTAGACACGCGAGAC	CAAGAGGGTGGTGGAGAGTA	50.0	55.0	58.0	58.1	1202-1383 bp	1.0	90.1
trnL-CAA-ycf2_p5	TGAAATGGTAGACACGCGAG	CAAGAGGGTGGTGGAGAGTA	50.0	55.0	58.0	58.1	1204-1385 bp	1.0	90.0