Hotspot

trnN-GUU-ndhF

SSC · 113321-114690 · 1370 bp

Back to Triplostegia

Pi 0.281

MarkerSeek score 83.30

Primer available yes

Label trnN-GUU-ndhF

Feature Metadata

label_name: trnN-GUU-ndhF
feature_type: igs
region: SSC
length_bp: 1370
variable_sites: 324
pi: 0.281
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 83.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ndhF_p1	CCCCGTAACTCTTCTTCAGC	TAGGAACTGGGAGTGGAAGG	55.0	55.0	58.0	58.0	1843-2092 bp	1.0	91.5
trnN-GUU-ndhF_p2	CCCGTAACTCTTCTTCAGCC	TAGGAACTGGGAGTGGAAGG	55.0	55.0	58.0	58.0	1842-2091 bp	1.0	91.5
trnN-GUU-ndhF_p3	CCCCGTAACTCTTCTTCAGC	GCGCCGGCAAATAATAGAAC	55.0	50.0	58.0	58.0	2495 bp	0.5	59.6
trnN-GUU-ndhF_p4	CCCGTAACTCTTCTTCAGCC	GCGCCGGCAAATAATAGAAC	55.0	50.0	58.0	58.0	2494 bp	0.5	59.6
trnN-GUU-ndhF_p5	GCCCCGTAACTCTTCTTCAG	GCGCCGGCAAATAATAGAAC	55.0	50.0	58.0	58.0	2496 bp	0.5	59.6