Hotspot

ycf2-trnL-CAA

IRb · 97700-98323 · 624 bp

Back to Triplostegia

Pi 0.169

MarkerSeek score 80.40

Primer available yes

Label ycf2-trnL-CAA

Feature Metadata

label_name: ycf2-trnL-CAA
feature_type: igs
region: IRb
length_bp: 624
variable_sites: 88
pi: 0.169
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2-trnL-CAA_p1	TGGCTTTTCCCGGATGAAAA	AAATGGTAGACACGCGAGAC	45.0	50.0	58.0	58.0	719-765 bp	1.0	91.9
ycf2-trnL-CAA_p2	TGGCTTTTCCCGGATGAAAA	TGAAATGGTAGACACGCGAG	45.0	50.0	58.0	58.0	721-767 bp	1.0	91.9
ycf2-trnL-CAA_p3	TGGCTTTTCCCGGATGAAAA	CCAACACTCCACCTTTGTCA	45.0	50.0	58.0	57.9	813-859 bp	1.0	91.6
ycf2-trnL-CAA_p4	CAAGAGGGTGGTGGAGAGTA	AAATGGTAGACACGCGAGAC	55.0	50.0	58.1	58.0	1202-1383 bp	1.0	90.1
ycf2-trnL-CAA_p5	CAAGAGGGTGGTGGAGAGTA	TGAAATGGTAGACACGCGAG	55.0	50.0	58.1	58.0	1204-1385 bp	1.0	90.0