Hotspot

psaJ-rpl33

LSC · 71467-71963 · 497 bp

Back to Triplostegia

Pi 0.00637

MarkerSeek score 65.00

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 497
variable_sites: 3
pi: 0.00637
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TGGCACCGGTACTAAGTACT	GAAATGCCCGTTGATACCCT	50.0	50.0	57.8	57.9	670-696 bp	1.0	81.2
psaJ-rpl33_p2	GTTCGGGTCTTTAGCAGGTC	GAAATGCCCGTTGATACCCT	55.0	50.0	58.3	57.9	645-671 bp	1.0	80.9
psaJ-rpl33_p3	GTGGCACCGGTACTAAGTAC	GAAATGCCCGTTGATACCCT	55.0	50.0	57.7	57.9	671-697 bp	1.0	80.7
psaJ-rpl33_p4	TGGCACCGGTACTAAGTACT	CGTTTCGGACACAACTGGTA	50.0	50.0	57.8	58.1	642-668 bp	1.0	80.6
psaJ-rpl33_p5	TTCGGGTCTTTAGCAGGTCT	GAAATGCCCGTTGATACCCT	50.0	50.0	58.4	57.9	644-670 bp	1.0	80.2