Hotspot

accD-psaI

LSC · 62304-62875 · 572 bp

Back to Triplostegia

Pi 0.0113

MarkerSeek score 68.80

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 572
variable_sites: 6
pi: 0.0113
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 68.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TCAGGTCCATGGTTTCTTTCC	CCATTGCAATTGCCGGAAAT	47.6	45.0	57.8	58.0	693-833 bp	1.0	75.2
accD-psaI_p2	CAGGTCCATGGTTTCTTTCCT	CCATTGCAATTGCCGGAAAT	47.6	45.0	57.8	58.0	692-832 bp	1.0	75.1
accD-psaI_p3	TCAGGTCCATGGTTTCTTTCC	AAGAAGCCATTGCAATTGCC	47.6	45.0	57.8	57.9	699-839 bp	1.0	74.6
accD-psaI_p4	CAGGTCCATGGTTTCTTTCCT	AAGAAGCCATTGCAATTGCC	47.6	45.0	57.8	57.9	698-838 bp	1.0	74.5
accD-psaI_p5	TCAGGTCCATGGTTTCTTTCC	AGGCACAAAAACAGAGGGAA	47.6	45.0	57.8	57.5	659-799 bp	1.0	71.8