Hotspot

petN-psbM

LSC · 30571-31775 · 1205 bp

Back to Triplostegia

Pi 0.00604

MarkerSeek score 65.90

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1205
variable_sites: 7
pi: 0.00604
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	CTAGTCCCTACCGCCTTTCT	50.0	55.0	58.2	58.2	1302-1348 bp	1.0	80.2
petN-psbM_p2	TAGTAAGTCTCGCTTGGGCT	TCTAGTCCCTACCGCCTTTC	50.0	55.0	58.2	58.2	1303-1349 bp	1.0	80.2
petN-psbM_p3	TAGTAAGTCTCGCTTGGGCT	TCCCTACCGCCTTTCTACTT	50.0	50.0	58.2	57.8	1298-1344 bp	1.0	80.1
petN-psbM_p4	TAGTAAGTCTCGCTTGGGCT	TTCTAGTCCCTACCGCCTTT	50.0	50.0	58.2	57.8	1304-1350 bp	1.0	80.0
petN-psbM_p5	TAGTAAGTCTCGCTTGGGCT	CATTCTAGTCCCTACCGCCT	50.0	55.0	58.2	58.3	1306-1352 bp	1.0	79.6