Hotspot

atpH-atpI

LSC · 14760-15931 · 1172 bp

Back to Triplostegia

Pi 0.018

MarkerSeek score 69.60

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1172
variable_sites: 21
pi: 0.018
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 69.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AGCCAATCCAGCAGCAATAA	TTTTTGCAACTTTAGCCGCG	45.0	45.0	57.9	58.5	1271-1276 bp	1.0	76.4
atpH-atpI_p2	CAGCAGCAATAACAGAAGCG	TTTTTGCAACTTTAGCCGCG	50.0	45.0	57.8	58.5	1263-1268 bp	1.0	76.2
atpH-atpI_p3	GCCAATCCAGCAGCAATAAC	TTTTTGCAACTTTAGCCGCG	50.0	45.0	57.8	58.5	1270-1275 bp	1.0	75.8
atpH-atpI_p4	AGCCAATCCAGCAGCAATAA	CCGCGGCTTATATAGGTGAA	45.0	50.0	57.9	57.3	1256-1261 bp	1.0	74.6
atpH-atpI_p5	CAGCAGCAATAACAGAAGCG	CCGCGGCTTATATAGGTGAA	50.0	50.0	57.8	57.3	1248-1253 bp	1.0	74.5