Hotspot

rpoC2

LSC · 27508-32112 · 4605 bp

Back to Tripidium

Pi 0.00116

MarkerSeek score 40.50

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4605
variable_sites: 8
pi: 0.00116
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	GGTTCACGATGCATTGCTTC	CCTACAGCTTCCCCCAATTC	50.0	55.0	58.1	57.9	209 bp	1.0	79.5
rpoC2_p2	GGTTCACGATGCATTGCTTC	ATTCGACCAGATCCCCATGA	50.0	50.0	58.1	58.2	193 bp	1.0	78.5
rpoC2_p3	ACGATGCATTGCTTCTCGAA	CCTACAGCTTCCCCCAATTC	45.0	55.0	58.3	57.9	204 bp	1.0	78.1
rpoC2_p4	ATTGGTTCACGATGCATTGC	CCTACAGCTTCCCCCAATTC	45.0	55.0	57.7	57.9	212 bp	1.0	78.0
rpoC2_p5	GGTTCACGATGCATTGCTTC	TACCTACAGCTTCCCCCAAT	50.0	50.0	58.1	57.7	211 bp	1.0	77.7