Hotspot

rpl16

LSC · 80296-81793 · 1498 bp

Back to Tripidium

Pi 0.00445

MarkerSeek score 51.90

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1498
variable_sites: 10
pi: 0.00445
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TCCAGAAAGAAAAACCAGGGG	AAAGTATCCCTCTTTCTATAAGAT	47.6	29.2	58.0	52.4	1600-1618 bp	1.0	41.0
rpl16_p2	TCCAGAAAGAAAAACCAGGGG	AAGTATCCCTCTTTCTATAAGATT	47.6	29.2	58.0	52.4	1599-1617 bp	1.0	41.0
rpl16_p3	TCCAGAAAGAAAAACCAGGGG	AGTATCCCTCTTTCTATAAGATTT	47.6	29.2	58.0	52.4	1598-1616 bp	1.0	41.0
rpl16_p4	TCCAGAAAGAAAAACCAGGGG	TCCCTCTTTCTATAAGATTTAATGA	47.6	28.0	58.0	53.3	1594-1612 bp	1.0	41.0
rpl16_p5	TCCAGAAAGAAAAACCAGGGG	AAGTATCCCTCTTTCTATAAGATTT	47.6	28.0	58.0	53.2	1599-1617 bp	1.0	41.0