Hotspot

ndhH

SSC · 117264-118445 · 1182 bp

Back to Tripidium

Pi 0.00451

MarkerSeek score 61.60

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: SSC
length_bp: 1182
variable_sites: 8
pi: 0.00451
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	TAGGAGGGCCAAAATGGGTA	TGCATTTTGATGAGCCTCGT	50.0	45.0	58.0	58.2	1356-1367 bp	1.0	79.0
ndhH_p2	CAGTAGGAGGGCCAAAATGG	TGCATTTTGATGAGCCTCGT	55.0	45.0	58.2	58.2	1359-1370 bp	1.0	77.2
ndhH_p3	CCAGTAGGAGGGCCAAAATG	TGCATTTTGATGAGCCTCGT	55.0	45.0	58.2	58.2	1360-1371 bp	1.0	77.2
ndhH_p4	GAGGGCCAAAATGGGTAGAA	TGCATTTTGATGAGCCTCGT	50.0	45.0	57.5	58.2	1353-1364 bp	1.0	74.9
ndhH_p5	TAGGAGGGCCAAAATGGGTA	TTGCATTTTGATGAGCCTCG	50.0	45.0	58.0	57.1	1357-1368 bp	1.0	73.0