Hotspot

rbcL-psaI

LSC · 58998-59936 · 939 bp

Back to Tripidium

Pi 0.0114

MarkerSeek score 51.50

Primer available yes

Label rbcL-psaI

Feature Metadata

label_name: rbcL-psaI
feature_type: igs
region: LSC
length_bp: 939
variable_sites: 16
pi: 0.0114
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psaI_p1	TGCAAATGGAGTGCTGAACT	CCAAGATAACTGGAAGCCCC	45.0	55.0	58.0	57.9	1630-1638 bp	1.0	80.5
rbcL-psaI_p2	GGCTTCAGGGGGTATTCATG	GTCATTGCAATTGCCGGAAA	55.0	45.0	58.0	57.9	1310-1318 bp	1.0	80.5
rbcL-psaI_p3	GGCTTCAGGGGGTATTCATG	CCAAGATAACTGGAAGCCCC	55.0	55.0	58.0	57.9	1844-1852 bp	1.0	80.4
rbcL-psaI_p4	TCTCATTATTGCCGCGACAA	CCAAGATAACTGGAAGCCCC	45.0	55.0	58.0	57.9	2134-2142 bp	1.0	80.3
rbcL-psaI_p5	TTGTCTCATTATTGCCGCGA	CCAAGATAACTGGAAGCCCC	45.0	55.0	58.0	57.9	2137-2145 bp	1.0	80.3