Hotspot

trnR-UCU-trnfM-CAU

LSC · 38880-39059 · 180 bp

Back to Tripidium

Pi 0.0222

MarkerSeek score 54.60

Primer available yes

Label trnR-UCU-trnfM-CAU

Feature Metadata

label_name: trnR-UCU-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 180
variable_sites: 6
pi: 0.0222
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-trnfM-CAU_p1	AGAAGACCTCTGTCCTATCCA	TCCCCGCTAAGAAGGTAGAG	47.6	55.0	56.9	57.9	275-303 bp	1.0	67.2
trnR-UCU-trnfM-CAU_p2	AGGTTTAGAAGACCTCTGTCCT	TCCCCGCTAAGAAGGTAGAG	45.5	55.0	57.9	57.9	281-309 bp	1.0	66.5
trnR-UCU-trnfM-CAU_p3	AGAAGACCTCTGTCCTATCCA	CCAACCCCCTTTTATCCACC	47.6	55.0	56.9	58.1	298-326 bp	1.0	66.3
trnR-UCU-trnfM-CAU_p4	AGGTTTAGAAGACCTCTGTCCT	CCAACCCCCTTTTATCCACC	45.5	55.0	57.9	58.1	304-332 bp	1.0	65.6
trnR-UCU-trnfM-CAU_p5	AGAAGACCTCTGTCCTATCCA	ACCCCCTTTTATCCACCAGT	47.6	50.0	56.9	58.2	295-323 bp	1.0	65.6