Hotspot

trnG-UCC-trnT-GGU

LSC · 14490-16438 · 1949 bp

Back to Tripidium

Pi 0.00239

MarkerSeek score 59.50

Primer available yes

Label trnG-UCC-trnT-GGU

Feature Metadata

label_name: trnG-UCC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 1949
variable_sites: 7
pi: 0.00239
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnT-GGU_p1	ACGAATCACACTTTTACCACT	ACCACTAAGTGAAAAGCCCT	38.1	45.0	55.5	56.7	2068-2074 bp	1.0	41.3
trnG-UCC-trnT-GGU_p2	ACGAATCACACTTTTACCACT	TGAACCGATGACTTATGCCT	38.1	45.0	55.5	56.6	2038-2044 bp	1.0	41.1
trnG-UCC-trnT-GGU_p3	ACGAATCACACTTTTACCACT	GACTTCTGTCTTACCAAGGCA	38.1	47.6	55.5	57.6	2096-2102 bp	1.0	40.9
trnG-UCC-trnT-GGU_p4	ACGAATCACACTTTTACCACT	TGACTTCTGTCTTACCAAGGC	38.1	47.6	55.5	57.6	2097-2103 bp	1.0	40.9
trnG-UCC-trnT-GGU_p5	ACGAATCACACTTTTACCACT	CCGATGACTTATGCCTTACCA	38.1	47.6	55.5	57.5	2034-2040 bp	1.0	40.4