Hotspot

trnG-GCC-trnfM-CAU

LSC · 13047-13548 · 502 bp

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Pi 0.0186

MarkerSeek score 73.30

Primer available yes

Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name: trnG-GCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 502
variable_sites: 14
pi: 0.0186
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnfM-CAU_p1	AATATAGGCCGTAGCAAGCG	CGAGGCTCATAACCTTGAGG	50.0	55.0	57.9	57.8	647-665 bp	1.0	81.8
trnG-GCC-trnfM-CAU_p2	AATATAGGCCGTAGCAAGCG	CAGTTTGGTAGCTCACGAGG	50.0	55.0	57.9	58.3	662-680 bp	1.0	81.3
trnG-GCC-trnfM-CAU_p3	AATATAGGCCGTAGCAAGCG	CAGTTTTCGGCCCTACAGAG	50.0	55.0	57.9	58.3	692-710 bp	1.0	81.2
trnG-GCC-trnfM-CAU_p4	AATATAGGCCGTAGCAAGCG	CTACAGAGCGGAGTAGAGCA	50.0	55.0	57.9	58.3	680-698 bp	1.0	80.8
trnG-GCC-trnfM-CAU_p5	AATATAGGCCGTAGCAAGCG	TACAGAGCGGAGTAGAGCAG	50.0	55.0	57.9	58.3	679-697 bp	1.0	80.8