Hotspot

trnS-GCU-psbD

LSC · 8427-9451 · 1025 bp

Back to Tripidium

Pi 0.00265

MarkerSeek score 65.30

Primer available yes

Label trnS-GCU-psbD

Feature Metadata

label_name: trnS-GCU-psbD
feature_type: igs
region: LSC
length_bp: 1025
variable_sites: 4
pi: 0.00265
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-psbD_p1	ATTAGCAATCCGCCGCTTTA	ATAAGCCGGACCATCCTACA	45.0	50.0	58.0	57.9	1127-1174 bp	1.0	82.5
trnS-GCU-psbD_p2	ATTAGCAATCCGCCGCTTTA	TAAGCCGGACCATCCTACAA	45.0	50.0	58.0	58.1	1126-1173 bp	1.0	82.2
trnS-GCU-psbD_p3	ATTAGCAATCCGCCGCTTTA	GCCGGACCATCCTACAAAAA	45.0	50.0	58.0	58.2	1123-1170 bp	1.0	81.9
trnS-GCU-psbD_p4	ATTAGCAATCCGCCGCTTTA	ACAAAAACGAAACGGTCCCT	45.0	45.0	58.0	58.2	1110-1157 bp	1.0	81.3
trnS-GCU-psbD_p5	GCTTTAGTCCACTCAGCCAT	ATAAGCCGGACCATCCTACA	50.0	50.0	57.6	57.9	1113-1160 bp	1.0	79.5