Hotspot

psbK-psbI

LSC · 7707-8101 · 395 bp

Back to Tripidium

Pi 0.00508

MarkerSeek score 59.70

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 395
variable_sites: 3
pi: 0.00508
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	CGTGGATGTTATGCCTGTCA	TTTTTACTCCTCACGCCCAG	50.0	50.0	58.0	57.8	581-588 bp	1.0	79.8
psbK-psbI_p2	GTTTGGCAAGCTGCTGTAAG	TTTTTACTCCTCACGCCCAG	50.0	50.0	57.9	57.8	532-539 bp	1.0	79.6
psbK-psbI_p3	TTAGCGTTTGTTTGGCAAGC	TTTTTACTCCTCACGCCCAG	45.0	50.0	58.2	57.8	541-548 bp	1.0	78.8
psbK-psbI_p4	ACCCGAAGCTTATGCCATTT	TTTTTACTCCTCACGCCCAG	45.0	50.0	57.9	57.8	611-618 bp	1.0	78.7
psbK-psbI_p5	TGGCAAGCTGCTGTAAGTTT	TTTTTACTCCTCACGCCCAG	45.0	50.0	58.3	57.8	529-536 bp	1.0	77.6