Hotspot

rps15

SSC · 117246-117524 · 279 bp

Back to Triphora

Pi 0.0397

MarkerSeek score 62.50

Primer available yes

Label rps15

Feature Metadata

label_name: rps15
feature_type: gene
region: SSC
length_bp: 279
variable_sites: 11
pi: 0.0397
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15_p1	GCAAGCTCGAACACATTGAG	ACATCCGTCCCAAGGTATCA	50.0	50.0	57.8	58.1	1397 bp	0.5	57.1
rps15_p2	GCAAGCTCGAACACATTGAG	ACAATCCTCGGTTCTTGGTG	50.0	50.0	57.8	57.8	1439 bp	0.5	56.6
rps15_p3	GCAAGCTCGAACACATTGAG	ACACAATCCTCGGTTCTTGG	50.0	50.0	57.8	57.8	1441 bp	0.5	56.6
rps15_p4	GCAAGCTCGAACACATTGAG	CACAATCCTCGGTTCTTGGT	50.0	50.0	57.8	57.8	1440 bp	0.5	56.6
rps15_p5	GCAAGCTCGAACACATTGAG	GCAAGCTCGAACACATTGAG	50.0	50.0	57.8	57.8	1358 bp	0.5	56.2