Hotspot

rpl16

LSC · 79658-81573 · 1916 bp

Back to Triphora

Pi 0.0324

MarkerSeek score 67.30

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1916
variable_sites: 59
pi: 0.0324
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	GCATATCGGCGATTACCAGT	ACGGAAAAGAGATTGCACGT	50.0	45.0	57.9	58.1	2363-2405 bp	1.0	83.8
rpl16_p2	TCTCAATTCCCGAGCAATCG	ACGGAAAAGAGATTGCACGT	50.0	45.0	58.1	58.1	2586-2628 bp	1.0	83.6
rpl16_p3	CTGTCTCAATTCCCGAGCAA	ACGGAAAAGAGATTGCACGT	50.0	45.0	57.9	58.1	2589-2631 bp	1.0	83.2
rpl16_p4	AACTGTCTCAATTCCCGAGC	ACGGAAAAGAGATTGCACGT	50.0	45.0	57.9	58.1	2591-2633 bp	1.0	83.2
rpl16_p5	TCGCACCAAAGATTCGAGTT	ACGGAAAAGAGATTGCACGT	45.0	45.0	57.8	58.1	2569-2611 bp	1.0	82.8