Hotspot

rps15-psaC

IRa · 117525-117824 · 300 bp

Back to Triphora

Pi 0.0705

MarkerSeek score 70.40

Primer available yes

Label rps15-psaC

Feature Metadata

label_name: rps15-psaC
feature_type: igs
region: IRa
length_bp: 300
variable_sites: 21
pi: 0.0705
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 70.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-psaC_p1	TTTCGCGAAGATCCAACTGA	ACATCCGTCCCAAGGTATCA	45.0	50.0	57.6	58.1	661-1307 bp	1.0	78.2
rps15-psaC_p2	TTTCGCGAAGATCCAACTGA	ACAATCCTCGGTTCTTGGTG	45.0	50.0	57.6	57.8	703-1349 bp	1.0	77.6
rps15-psaC_p3	TTTCGCGAAGATCCAACTGA	ACACAATCCTCGGTTCTTGG	45.0	50.0	57.6	57.8	705-1351 bp	1.0	77.6
rps15-psaC_p4	TTTCGCGAAGATCCAACTGA	CACAATCCTCGGTTCTTGGT	45.0	50.0	57.6	57.8	704-1350 bp	1.0	77.6
rps15-psaC_p5	TTTCGCGAAGATCCAACTGA	GCAAGCTCGAACACATTGAG	45.0	50.0	57.6	57.8	622 bp	0.5	54.5