Hotspot

infA-rps8

LSC · 78472-78529 · 58 bp

Back to Triphora

Pi 0.0517

MarkerSeek score 70.70

Primer available yes

Label infA-rps8

Feature Metadata

label_name: infA-rps8
feature_type: igs
region: LSC
length_bp: 58
variable_sites: 3
pi: 0.0517
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
infA-rps8_p1	CCGTTGGGAAGTGATTCAGT	AGCTCGACTAGAAAAGATTGGAG	50.0	43.5	57.8	58.0	163 bp	1.0	56.0
infA-rps8_p2	ACCGTTGGGAAGTGATTCAG	AGCTCGACTAGAAAAGATTGGAG	50.0	43.5	57.8	58.0	164 bp	1.0	55.9
infA-rps8_p3	ACCGTTGGGAAGTGATTCAG	AGCTCGACTAGAAAAGATTGGA	50.0	40.9	57.8	56.9	164 bp	1.0	55.4
infA-rps8_p4	CCGTTGGGAAGTGATTCAGT	AGCTCGACTAGAAAAGATTGGA	50.0	40.9	57.8	56.9	163 bp	1.0	55.4
infA-rps8_p5	GGAACATACCGTTGGGAAGT	AGCTCGACTAGAAAAGATTGGAG	50.0	43.5	57.5	58.0	171 bp	1.0	53.5