Hotspot

psbE-petL

LSC · 61645-62766 · 1122 bp

Back to Triphora

Pi 0.021

MarkerSeek score 69.60

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1122
variable_sites: 23
pi: 0.021
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 69.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	TGACAAATAACCAACCCGCA	GGGTTATAGTTGAAGCAGCCA	45.0	47.6	58.0	58.0	1292-1307 bp	1.0	75.0
psbE-petL_p2	CAACCCGCAATGAATAGGGA	GGGTTATAGTTGAAGCAGCCA	50.0	47.6	57.9	58.0	1281-1296 bp	1.0	74.8
psbE-petL_p3	AGAACGTTCTCCTGTGCTTC	GGGTTATAGTTGAAGCAGCCA	50.0	47.6	57.8	58.0	1204-1219 bp	1.0	74.1
psbE-petL_p4	GTTCTCCTGTGCTTCCAGAC	GGGTTATAGTTGAAGCAGCCA	55.0	47.6	58.2	58.0	1199-1214 bp	1.0	73.8
psbE-petL_p5	ACCAACCCGCAATGAATAGG	GGGTTATAGTTGAAGCAGCCA	50.0	47.6	58.2	58.0	1283-1298 bp	1.0	73.3