Hotspot

petA-psbJ

LSC · 59933-60873 · 941 bp

Back to Triphora

Pi 0.0269

MarkerSeek score 61.90

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 941
variable_sites: 25
pi: 0.0269
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCATCTGTGATTTTCGCAC	GGGTTGGGTTCATCTCTGTA	50.0	50.0	58.1	56.5	1047-1081 bp	1.0	70.2
petA-psbJ_p2	GGCATCTGTGATTTTCGCAC	GGGTTGGGTTCATCTCTGTAA	50.0	47.6	58.1	57.3	1047-1081 bp	1.0	68.2
petA-psbJ_p3	TCCATTACGAGTTCAAGGCC	GGGTTGGGTTCATCTCTGTA	50.0	50.0	57.6	56.5	1080-1114 bp	1.0	67.7
petA-psbJ_p4	CCATTACGAGTTCAAGGCCT	GGGTTGGGTTCATCTCTGTA	50.0	50.0	57.6	56.5	1079-1113 bp	1.0	67.7
petA-psbJ_p5	TGGCATCTGTGATTTTCGCA	GGGTTGGGTTCATCTCTGTA	45.0	50.0	58.5	56.5	1048-1082 bp	1.0	67.2