Hotspot

atpB-rbcL

LSC · 51169-52022 · 854 bp

Back to Triphora

Pi 0.0244

MarkerSeek score 61.50

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 854
variable_sites: 20
pi: 0.0244
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	GTCCGATTTTTCCTCCACGA	GTCCACACAGTTGTCCATGT	50.0	50.0	57.9	58.0	1574-1643 bp	1.0	82.0
atpB-rbcL_p2	TCCGATTTTTCCTCCACGAC	GTCCACACAGTTGTCCATGT	50.0	50.0	57.9	58.0	1573-1642 bp	1.0	82.0
atpB-rbcL_p3	GTCCGATTTTTCCTCCACGA	ACGAAACGATCTCTCCAACG	50.0	50.0	57.9	58.0	2018-2087 bp	1.0	81.5
atpB-rbcL_p4	TCCGATTTTTCCTCCACGAC	ACGAAACGATCTCTCCAACG	50.0	50.0	57.9	58.0	2017-2086 bp	1.0	81.5
atpB-rbcL_p5	AGTCCGATTTTTCCTCCACG	ACGAAACGATCTCTCCAACG	50.0	50.0	57.9	58.0	2019-2088 bp	1.0	81.4