Hotspot

trnM-CAU-atpE

LSC · 49096-49270 · 175 bp

Back to Triphora

Pi 0.0629

MarkerSeek score 71.60

Primer available yes

Label trnM-CAU-atpE

Feature Metadata

label_name: trnM-CAU-atpE
feature_type: igs
region: LSC
length_bp: 175
variable_sites: 11
pi: 0.0629
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU-atpE_p1	ATGGAGACCCTTTTGTGGTG	CGAGCTAGGACACGAGTAGA	50.0	55.0	57.7	58.1	319-321 bp	1.0	79.9
trnM-CAU-atpE_p2	ATGGAGACCCTTTTGTGGTG	AGGACACGAGTAGAAGCTGT	50.0	50.0	57.7	58.1	313-315 bp	1.0	79.8
trnM-CAU-atpE_p3	TATTGCTTTCATACGGCGGG	CGAGCTAGGACACGAGTAGA	50.0	55.0	58.4	58.1	269-270 bp	1.0	79.5
trnM-CAU-atpE_p4	TATTGCTTTCATACGGCGGG	AGGACACGAGTAGAAGCTGT	50.0	50.0	58.4	58.1	263-264 bp	1.0	79.3
trnM-CAU-atpE_p5	ATGGAGACCCTTTTGTGGTG	GGACACGAGTAGAAGCTGTC	50.0	55.0	57.7	57.7	312-314 bp	1.0	78.3