Hotspot

atpH-atpI

LSC · 13615-14259 · 645 bp

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Pi 0.0176

MarkerSeek score 71.80

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 645
variable_sites: 11
pi: 0.0176
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 71.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	TACCTTGACCAATCCCAGGT	AACTTTGGCTGCGGCTTATA	50.0	45.0	57.9	57.9	775-782 bp	1.0	81.8
atpH-atpI_p2	TCCGATAGAAGCAAGCCCTA	AACTTTGGCTGCGGCTTATA	50.0	45.0	57.9	57.9	756-763 bp	1.0	81.6
atpH-atpI_p3	AACGGAAGCAGCAGAAATCA	AACTTTGGCTGCGGCTTATA	45.0	45.0	58.1	57.9	717-724 bp	1.0	81.6
atpH-atpI_p4	AGCCAATCCAGCAGCAATAA	AACTTTGGCTGCGGCTTATA	45.0	45.0	57.9	57.9	735-742 bp	1.0	81.2
atpH-atpI_p5	CCGATAGAAGCAAGCCCTAC	AACTTTGGCTGCGGCTTATA	55.0	45.0	57.8	57.9	755-762 bp	1.0	81.0