Hotspot

psbI-trnS-GCU

LSC · 7909-8077 · 169 bp

Back to Triphora

Pi 0.0617
MarkerSeek score 73.70
Primer available yes
Label psbI-trnS-GCU

Feature Metadata

label_name
psbI-trnS-GCU
feature_type
igs
region
LSC
length_bp
169
variable_sites
10
pi
0.0617
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
73.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbI-trnS-GCU_p1 AATCCTGGGCGTGAAGAAAA TGGACTAAAGCGTCGGATTG 45.0 50.0 57.7 58.0 264-269 bp 1.0 82.8
psbI-trnS-GCU_p2 ATCCTGGGCGTGAAGAAAAA TGGACTAAAGCGTCGGATTG 45.0 50.0 57.7 58.0 263-268 bp 1.0 82.8
psbI-trnS-GCU_p3 ATCCTGGGCGTGAAGAAAAA GTGGACTAAAGCGTCGGATT 45.0 50.0 57.7 58.0 264-269 bp 1.0 82.8
psbI-trnS-GCU_p4 AATCCTGGGCGTGAAGAAAA GTGGACTAAAGCGTCGGATT 45.0 50.0 57.7 58.0 265-270 bp 1.0 82.7
psbI-trnS-GCU_p5 CCTGGGCGTGAAGAAAAAGA TGGACTAAAGCGTCGGATTG 50.0 50.0 58.4 58.0 261-266 bp 1.0 82.0