Hotspot

accD

LSC · 60336-61979 · 1644 bp

Back to Triosteum

Pi 0.087

MarkerSeek score 83.00

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1644
variable_sites: 234
pi: 0.087
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 83.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	GGTAGTGGTCTCGCTTTCAG	CCCATTGCTACGGGAATACC	55.0	55.0	58.0	58.1	923-2114 bp	0.75	81.1
accD_p2	ACAGGTCTACAGGGGTACAC	CCATTGCAATTGCCGGAAAT	55.0	45.0	58.1	58.0	2780 bp	0.25	49.3
accD_p3	GTTACAGGGGTACAGGGCTA	CCATTGCAATTGCCGGAAAT	55.0	45.0	58.1	58.0	2971 bp	0.25	48.7
accD_p4	TTACAGGGGTACAGGGCTAC	CCATTGCAATTGCCGGAAAT	55.0	45.0	58.1	58.0	2970 bp	0.25	48.7
accD_p5	ACAGGTCTACAGGGGTACAC	CGGGAGGAGGAAATTCGTTT	55.0	50.0	58.1	57.9	2244 bp	0.25	48.6