Hotspot

trnN-GUU-trnR-ACG

IRa · 132033-132237 · 205 bp

Back to Triosteum

Pi 0.156

MarkerSeek score 73.60

Primer available yes

Label trnN-GUU-trnR-ACG

Feature Metadata

label_name: trnN-GUU-trnR-ACG
feature_type: igs
region: IRa
length_bp: 205
variable_sites: 58
pi: 0.156
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-trnR-ACG_p1	ATACCCACACCAGCCTTTTG	CTCGACGCCAGGATGTTAAA	50.0	50.0	58.1	58.0	1004-1455 bp	0.75	72.3
trnN-GUU-trnR-ACG_p2	ATGGCTATTGGTCGAACGAC	CTCGACGCCAGGATGTTAAA	50.0	50.0	58.1	58.0	1233-1540 bp	0.5	62.5
trnN-GUU-trnR-ACG_p3	CTTCTTCCTCTTCAACGCGA	CTCGACGCCAGGATGTTAAA	50.0	50.0	57.9	58.0	1136-1442 bp	0.5	61.9
trnN-GUU-trnR-ACG_p4	TCTTCTTCCTCTTCAACGCG	CTCGACGCCAGGATGTTAAA	50.0	50.0	57.9	58.0	1137-1443 bp	0.5	61.9
trnN-GUU-trnR-ACG_p5	ATGGCTATTGGTCGAACGAC	CCTTGTTCCTACATGACCCG	50.0	55.0	58.1	58.0	1565-1872 bp	0.5	61.8