Hotspot

rpl32-trnL-UAG

SSC · 115163-115966 · 804 bp

Back to Triosteum

Pi 0.0252

MarkerSeek score 69.20

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 804
variable_sites: 37
pi: 0.0252
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 69.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	TGTGCGACAAACAAATAAGTCA	AGCGTGTCTACCGATTTCAC	36.4	50.0	57.1	58.0	829-889 bp	1.0	64.1
rpl32-trnL-UAG_p2	TGTGCGACAAACAAATAAGTCA	CAGCGTGTCTACCGATTTCA	36.4	50.0	57.1	58.0	830-890 bp	1.0	64.0
rpl32-trnL-UAG_p3	TGTGCGACAAACAAATAAGTCA	TCCTAAGAGCAGCGTGTCTA	36.4	50.0	57.1	57.9	839-899 bp	1.0	63.0
rpl32-trnL-UAG_p4	TGTGCGACAAACAAATAAGTCA	CCTAAGAGCAGCGTGTCTAC	36.4	55.0	57.1	57.8	838-898 bp	1.0	62.5
rpl32-trnL-UAG_p5	TGTGCGACAAACAAATAAGTCA	CTAAGAGCAGCGTGTCTACC	36.4	55.0	57.1	57.8	837-897 bp	1.0	62.5