Hotspot

infA-rps8

LSC · 82952-83140 · 189 bp

Back to Triosteum

Pi 0.0549

MarkerSeek score 68.90

Primer available yes

Label infA-rps8

Feature Metadata

label_name: infA-rps8
feature_type: igs
region: LSC
length_bp: 189
variable_sites: 15
pi: 0.0549
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.78
markerseek_score: 68.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
infA-rps8_p1	AATGAACGCGGAACATACCA	GCTGCAGTCCTCATTGATCA	45.0	50.0	57.9	58.0	926-985 bp	1.0	85.8
infA-rps8_p2	TGAACGCGGAACATACCATT	GCTGCAGTCCTCATTGATCA	45.0	50.0	57.9	58.0	924-983 bp	1.0	85.8
infA-rps8_p3	TGAACGCGGAACATACCATT	TGCTGCAGTCCTCATTGATC	45.0	50.0	57.9	58.0	925-984 bp	1.0	85.8
infA-rps8_p4	TTTCGAGTCCTTGTTGCGAA	GCTGCAGTCCTCATTGATCA	45.0	50.0	58.1	58.0	1081-1140 bp	0.5	63.6
infA-rps8_p5	TTTCGAGTCCTTGTTGCGAA	TGCTGCAGTCCTCATTGATC	45.0	50.0	58.1	58.0	1082-1141 bp	0.5	63.6