Hotspot

trnP-GGG-psaJ

LSC · 70446-70940 · 495 bp

Back to Triosteum

Pi 0.0734

MarkerSeek score 83.50

Primer available yes

Label trnP-GGG-psaJ

Feature Metadata

label_name: trnP-GGG-psaJ
feature_type: igs
region: LSC
length_bp: 495
variable_sites: 69
pi: 0.0734
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 83.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-GGG-psaJ_p1	AAAACAAACGCGCTACCAAG	GGAGAGATATGTTTTTAGATCTCGC	45.0	40.0	57.9	57.4	513-587 bp	1.0	52.0
trnP-GGG-psaJ_p2	CAAAACAAACGCGCTACCAA	GGAGAGATATGTTTTTAGATCTCGC	45.0	40.0	58.2	57.4	514-588 bp	1.0	52.0
trnP-GGG-psaJ_p3	TTGTACCCAAAACAAACGCG	GGAGAGATATGTTTTTAGATCTCGC	45.0	40.0	57.8	57.4	521-595 bp	1.0	52.0
trnP-GGG-psaJ_p4	TACCCAAAACAAACGCGCTA	GGAGAGATATGTTTTTAGATCTCGC	45.0	40.0	58.4	57.4	518-592 bp	1.0	52.0
trnP-GGG-psaJ_p5	AAAACAAACGCGCTACCAAG	GAGAGATATGTTTTTAGATCTCGCA	45.0	36.0	57.9	56.8	512-586 bp	1.0	52.0