Hotspot

ycf4-cemA

LSC · 63820-64772 · 953 bp

Back to Triosteum

Pi 0.014

MarkerSeek score 70.50

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 953
variable_sites: 23
pi: 0.014
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 70.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TTGGCCTATTTCTTGCGTGT	GAGAAATCCACCAGGGCAAA	45.0	50.0	58.1	58.1	626-1070 bp	1.0	84.5
ycf4-cemA_p2	AAAAAGCTGCTGAATTGGCC	GAGAAATCCACCAGGGCAAA	45.0	50.0	57.8	58.1	640-1084 bp	1.0	83.9
ycf4-cemA_p3	AAAGCTGCTGAATTGGCCTA	GAGAAATCCACCAGGGCAAA	45.0	50.0	57.8	58.1	638-1082 bp	1.0	83.5
ycf4-cemA_p4	TTGGCCTATTTCTTGCGTGT	ATCCACCAGGGCAAAAAGAC	45.0	50.0	58.1	58.4	621-1065 bp	1.0	82.2
ycf4-cemA_p5	GGCCATTCCTTTGACTCGTA	GAGAAATCCACCAGGGCAAA	50.0	50.0	57.6	58.1	693-1137 bp	1.0	82.0