Hotspot

trnF-GAA-ndhJ

LSC · 50003-50711 · 709 bp

Back to Triosteum

Pi 0.0128

MarkerSeek score 69.10

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 709
variable_sites: 16
pi: 0.0128
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 69.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	TGGATAGGATGGCCTTTACG	55.0	50.0	58.0	56.8	837-853 bp	1.0	72.8
trnF-GAA-ndhJ_p2	GGGATAGCTCAGCTGGTAGA	GGATAGGATGGCCTTTACGT	55.0	50.0	58.0	56.8	836-852 bp	1.0	72.8
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	TGGATAGGATGGCCTTTACG	50.0	50.0	58.1	56.8	801-817 bp	1.0	72.6
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	GGATAGGATGGCCTTTACGT	50.0	50.0	58.1	56.8	800-816 bp	1.0	72.6
trnF-GAA-ndhJ_p5	CTGGTAGAGCAGAGGACTGA	TGGATAGGATGGCCTTTACG	55.0	50.0	57.9	56.8	825-841 bp	1.0	71.8